Binding profiles and cytokine-inducing effects of fish rhamnose-binding lectins on Burkitt’s lymphoma Raji cells     

Masahiro Hosono  Shigeki Sugawara  Atsushi Matsuda  Takeo Tatsuta  Yasuhiro Koide  Imtiaji Hasan  Yasuhiro Ozeki  Kazuo Nitta 《Fish physiology and biochemistry》2014,40(5):1559-1572

Rhamnose-binding lectin (RBL) is one of the animal lectin categories which take part in the innate immune responses of fish. Osmerus lanceolatus lectin (OLL) from shishamo smelt eggs is an RBL composed of two tandem-repeated domains, both of which are considered to be a carbohydrate-recognition domain. SAL, catfish (Silurus asotus) egg RBL composed of three domains, binds to Burkitt’s lymphoma Raji cells through globotriaosylceramide (Gb3) carbohydrate chain and to reduce cell size and growth by altering membrane composition without causing cell death. In this experiment, we tried to compare the binding effects of these two RBLs on Raji cells. Flow cytometric and fluorescence microscopic analyses revealed that OLL also directly bound to and shrunk Raji cells with ten times less reactivity than SAL but reduced cell growth with decreasing cell viability. Anti-Gb3 antibody completely blocked the binding of SAL to Raji cells but not that of OLL. In addition, the direct bindings of OLL and SAL to Raji cells were comparably inhibited by melibiose, but lactose was more effective inhibitor for the binding of OLL than that of SAL. These results suggest that OLL has slightly different cell-binding property compared with SAL and binds not only to Gb3 but also to the other carbohydrate receptor-bearing β-galactoside chains. The quantitative RT-PCR analysis revealed that SAL induced the expression of TNF-α but not of IFN-γ, IL-1β, and IL-10. Thus, SAL-induced cytostatic effect on Raji cells might be partially caused by TNF-α-mediated signaling pathway.  相似文献   

Effect of Soil Sample Size on Saturated Soil Hydraulic Conductivity     

Roya Jafari  Mohsen Hossein-Alizadeh  Hasan Rezaii-Moghadam 《Communications in Soil Science and Plant Analysis》2017,48(8):908-919

Saturated hydraulic conductivity (K_sat) is regarded as a key soil physical variable to determine soil infiltration rate, percolation depth and other hydrological processes. The purpose of this study was to determine the best soil sampling’s ring size for measuring K_sat. For this purpose, 25 rings with five different diameters (2, 3, 4, 5 and 6 inch) and with the same depth of 20 cm (five replicates) were hammered in close vicinity to each other into the ground of undisturbed loess deposits of a small farm to measure K_sat and bulk density. Hydraulic conductivity was measured at three constant loads of 22, 27 and 32 cm. The results showed that the ring with the internal diameter of 6 inches had the minimum variation coefficient and maximum K_sat for all the three hydraulic heads. In addition, the bulk density of this ring size was less than the other rings.  相似文献   

Phytoremediation Potential of Vetiver Grass (<Emphasis Type="Italic">Vetiveria zizanioides</Emphasis>) for Treatment of Metal-Contaminated Water     

Ashton Lim Suelee  Sharifah Nur Munirah Syed Hasan  Faradiella Mohd Kusin  Ferdaus Mohamat Yusuff  Zelina Zaiton Ibrahim 《Water, air, and soil pollution》2017,228(4):158

Phytoremediation using vetiver grass (Vetiveria zizanioides) has been regarded as an effective technique for removing contaminants in polluted water. This study was conducted to assess the removal efficiency of heavy metals (Cu, Fe, Mn, Pb, Zn) using vetiver grass (VG) at different root lengths and densities and to determine metals uptake rate by plant parts (root and shoot) between treatments (low and high concentration). Removal efficiency for heavy metals in water by VG is ranked in the order of Fe>Pb>Cu>Mn>Zn. Results showed that VG was effective in removing all the heavy metals, but removals greatly depend on root length, plant density and metal concentration. Longer root length and higher density showed greater removals of heavy metals due to increased surface area for metal absorption by plant roots. Results also demonstrated significant difference of heavy metals uptake in plant parts at different concentrations indicating that root has high tolerance towards elevated concentration of heavy metals. However, the effects were less significant in plant shoot suggesting that metals uptake were generally higher in root than in shoot. The findings have shown potential of VG in phytoremediation for heavy metals removal in water thus providing significant implication for treatment of metal-contaminated water.  相似文献   

Screening for bovine leukocyte adhesion deficiency,deficiency of uridine monophosphate synthase,complex vertebral malformation,bovine citrullinaemia,and factor XI deficiency in Holstein cows reared in Turkey     

Hasan Meydan  Mehmet A Yildiz  J?rgen S Agerholm 《Acta veterinaria Scandinavica》2010,52(1):56

Background

Bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FXID) are autosomal recessive hereditary disorders, which have had significant economic impact on dairy cattle breeding worldwide. In this study, 350 Holstein cows reared in Turkey were screened for BLAD, DUMPS, CVM, BC and FXID genotypes to obtain an indication on the importance of these defects in Turkish Holsteins.

Methods

Genomic DNA was obtained from blood and the amplicons of BLAD, DUMPS, CVM, BC and FXID were obtained by using PCR. PCR products were digested with TaqI, AvaI and AvaII restriction enzymes for BLAD, DUMPS, and BC, respectively. These digested products and PCR product of FXID were analyzed by agarose gel electrophoresis stained with ethidium bromide. CVM genotypes were detected by DNA sequencing. Additionally, all genotypes were confirmed by DNA sequencing to determine whether there was a mutant allele or not.

Results

Fourteen BLAD, twelve CVM and four FXID carriers were found among the 350 Holstein cows examined, while carriers of DUMPS and BC were not detected. The mutant allele frequencies were calculated as 0.02, 0.017, and 0.006 for BLAD, CVM and FXID, respectively with corresponding carrier prevalence of 4.0% (BLAD), 3.4% (CVM) and 1.2% (FXID).

Conclusion

This study demonstrates that carriers of BLAD, CVM and FXID are present in the Turkish Holstein population, although at a low frequency. The actual number of clinical cases is unknown, but sporadic cases may appear. As artificial insemination is widely used in dairy cattle breeding, carriers of BLAD, CVM and FXID are likely present within the population of breeding sires. It is recommended to screen breeding sires for these defective genes in order to avoid an unwanted spread within the population.  相似文献   

Nitrate Poisoning in Horses Associated With Ingestion of Forage and Alfalfa     

Hasan H. Oruc  Ahmet Akkoc  Ilknur Uzunoglu  Engin Kennerman 《Journal of Equine Veterinary Science》2010

This report describes nitrate poisoning observed in thoroughbred mares. Nine horses died at a farm in Bursa, Turkey, between February and April of 2008. Five of them were pregnant. Clinical findings developed over a short period and included severe abdominal pain, limited response to analgesics and antispasmodics, diarrhea, shallow and rapid breathing, tachycardia, blue-brown discoloration of the mucosal membranes, tremors, ataxia, convulsions before death, and abortion in the pregnant mares. The main postmortem finding was chocolate brown–colored blood as well as congestion and inflammation of the intra-abdominal organs. Uterine rupture, colonic ruptures, and torsio uteri were seen in the pregnant mares. Stomach and intestinal ruptures were seen in four nonpregnant mares. High nitrate concentrations were determined in some grass and alfalfa samples used to feed the animals. Nitrate poisoning is considered to be very rare in horses, but, in this case, the clinical symptoms, postmortem findings, and nitrate levels in the collected samples supported nitrate and nitrite poisoning as the cause of deaths.  相似文献   

Cold storage of oil rose (Rosa damascena Mill.) flowers     

Soner Kazaz  Sabri Erbas  Hasan Baydar  Tuba Dilmacunal  Mehmet Ali Koyuncu 《Scientia Horticulturae》2010

Oil rose flowers were stored at 0 °C in four different packaging materials [plastic box + stretch film (PB + SF), Xtend^®, Smartbag^® and polyethylene (PE)] for 60 days. During storage, weight loss, O₂ and CO₂ concentrations in the packages, petal color and sensorial attributes were investigated besides essential oil content and composition. Storage duration and packages had significant (p < 0.01) effects on weight loss. At the end of storage, the lowest weight loss was in PE package (1.696%) whereas the highest weight loss was in Xtend^® (10.081%). The essential oil content was significantly (p < 0.01) affected by storage duration and packages. In addition, the essential oil contents obtained from all packages for a storage period of 10 days and the essential oil contents obtained from unstored (control) petals were included in the same group. At the end of storage, the essential oil contents decreased by 91.3, 57.7, 80.0 and 64.3% in PB + SF, Xtend^®, Smartbag^® and PE packages, respectively as compared to control. In addition, storage duration and package types significantly (p < 0.01) affected petal color, O₂ and CO₂ concentrations in the packages and sensorial scores. The concentration of citronellol, a main component of rose oil, increased in all packages during storage of 10 days in comparison to the control group while it varied in other storage durations and package types. However, nerol and geraniol were lower than the control group during storage while concentrations of nonadecane, heneicosane and eicosane were higher. In conclusion, loss of oil yield and quality, due to various reasons and particularly due to fermentation in oil rose from the harvest of petals to their distillation, can be minimized with storage of petals in all package types for up to 10 days.  相似文献   

Ecological disturbances due to high cutback in the green infrastructure of Karachi: Analyses of public perception about associated health problems     

Salman Qureshi  Syed Jamil Hasan Kazmi  Jürgen H. Breuste 《Urban Forestry & Urban Greening》2010,9(3):187-198

This paper evaluates the changes to the green infrastructure of the megacity of Karachi, Pakistan, occurring during a period of rapid development, involving extensive loss of trees and green spaces since 2000, occurring as a consequence of road widening and improvements to the city's transport infrastructure, aggravated by a series of cyclones, involving further tree loss and which has led to numerous ecological problems in the city, assessing how the effect of the reduction of Karachi's green infrastructure has impacted both the reality and the perception of human health conditions. It uses medium-resolution satellite images classified for land use and land cover (LULC) data extraction and detailed field surveys to map the extent of change; structured questionnaires are used to identify the perception of selected, targeted groups regarding the state of Karachi's urban green infrastructure and perceived human health conditions. Findings indicate that the public perception of green space functionality or presence does not equate with the remote sensing and field mapping results, which show a dramatic loss. The need to develop a comprehensive urban greening strategy, which considers the needs and priorities of the population, is identified. The impact of such a strategy in terms of increased frequency of visits to green sites and associated increase in the physical activity of the people to improve overall physical health is discussed in relation to the general development of Karachi.  相似文献   

Proteome profiling of the compatible interaction between wheat and stripe rust     

Yahya?Emin?Demirci  Cihan?Inan  Asl?han?Günel  Dilara?Maytalman  Zafer?Mert  A.?Tar?k?Baykal  ?enay?Vural?Korkut  Nazl??Arda  Semra?Hasan?ebiEmail authorView author&#;s OrcID profile 《European journal of plant pathology / European Foundation for Plant Pathology》2016,145(4):941-962

Over the last decade, comparative molecular profiling studies between compatible and incompatible plant-pathogen interactions have shown that susceptible response of the host to a pathogen requires factors that promote disease development. In this study, we examined proteome profiles during a compatible interaction between wheat and stripe rust. A 2D-LC system (ProteomeLab PF2D) was used for protein separation and to compare the proteome from infected and control samples. More than 700 protein peaks at each time point were compared between pathogen- and mock-inoculated samples. Selected proteins, with significant differences in abundance were identified by nanoLC-ESI- MS/MS and generated spectra were searched against the wheat protein databases from UniProt, and NCBI and the Puccinia database from The Broad Institute. In total, the identified proteins comprised of 62 % wheat and 38 % Pst proteins. All identified proteins were searched by bioinformatics-based algorithms to detect their subcellular localization and signal peptide motifs which have the potential to catch the candidate effector proteins. The wheat proteins were classified based on their function. Although a compatible interaction, many wheat proteins, such as antioxidants, PRs and cold-responsive proteins, are implicated in defense and stress tolerance. On the pathogen side, 64 proteins were identified, and included some important pathogenicity proteins that can play role in pathogen virulence and suppress the host defense. In addition, we discovered that nine proteins have a signal sequence and three of the hypothetical fungal proteins, PGTG_11681T0, PGTG_07231T0 and CBH50687.1, have been tentatively identified as candidate effectors.  相似文献   

Recent Advances in the Use of Plant Pathogens as Biocontrol Agents of Weeds     

S. Hasan 《国际虫害防治杂志》2013,59(4):437-443

Abstract

Among various organisms attacking skeleton weed, Chondrilla juncea, in Europe, a rust fungus, Puccinia chondrillina, and two powdery mildews, Erysiphe cichoracearum and Leveillula taurica f. sp. chondrillae were found to be the most damaging. P. chondrillina was shown to be specific to Chondrilla and has recently been introduced into Australia where skeleton weed is important in wheat cultivation. It has already spread throughout the weed infestations and is beginning to reduce plant populations. Encouraged by the result of the Chondrilla rust, the possible use of Puccinia xanthii for the control of Xanthium strumarium and X. spinosum has also been studied. Current research on the possible use of phytopathogens in the biological control of some other weeds is reviewed.  相似文献   

Regional inflation persistence in Turkey     

Hasan Engin Duran  Burak Dindarolu 《Growth and change》2021,52(1):460-491

The purpose of the current study is to investigate the degree of inflation persistence, its geographical variation, sources of cross‐regional variation, and presence of geographical/sectoral aggregation bias in national monetary policy. Our data set covers 26 NUTS‐2 level Turkish regions and monthly CPI inflation over the period 2003–2019. We first estimate the degree of regional inflation persistence by autoregressive regressions, check its robustness against the presence of structural breaks (by Bai–Perron's algorithm) and nonlinearities (by Markovian Regime Switching regressions). Second, we examine the possibility of geographical and sectoral aggregation bias. Third, we investigate the cross‐regional determinants of inflation persistence by panel data analysis, employing hybrid‐effects spatial panel regressions. We analyze the direct and indirect effects of the determinants and test for regional spillover effects. Three main results are obtained. First, estimated persistence degrees are heterogeneous across regions. The geographical pattern is empirically robust against structural breaks and nonlinearities. We find that inflation persistence is distributed in a spatially correlated manner. Second, when sectoral and regional aggregation bias is tested, only sectoral aggregation indicates a considerable level of bias. Third, we find that the presence of large firms in the region and a higher share of agricultural output in GDP leads to lower persistence, while an increased share of industrial output, and increased trade volume leads to higher inflation persistence. Moreover, we find spatial spillovers of price variability evident in regression analysis. From a policy standpoint, it is required that structural policy programs are targeted to maintain flexibility in the regions where persistence is high (i.e., providing market entry/exit, institutional quality, policy credibility, stimulation of SMEs). Moreover, sectors that have high persistence, such as Hotels and Restaurants (persistence degree 0.55) and Health Services (0.39) should be weighted more in CPI calculations.  相似文献